Instructors: Theodore M. Nelson, Christopher E. Mason, Karolina Sienkiewicz
Lecture Summary: File formats describe the raw information available to analyze and interpret in scientific studies. In this video, we review the essential file formats underlying Single-Molecule Sequencing, including FAST5, POD5, BLOW5, SLOW5, FASTQ, SAM, BAM, and VCF. During the presentation, we relate to relevant concepts in genomic analysis (SNPs) and epitranscriptomics (M6A). Students proceeded through workshop style Google Colab notebooks.
Course Summary: Sequencing-based research has become the dominant investigative practice within the biological sciences. Single-molecule sequencing: training, methods, and applications, responds to this development by providing a hands-on and in-depth introduction to sequencing for students, from Sanger to third-generation technologies. Utilizing an alternating lecture-lab schedule, students are introduced to fundamental basic principles of DNA & RNA science, progressing to cutting-edge library preparation and sequencing analysis techniques with Illumina and Nanopore technologies. Students will have the opportunity to perform direct-RNA sequencing samples on research samples, and experience first-hand the ethical implications of this data. Relevant concepts in biology and computer science will be addressed.
GitHub Link: github.com/Theo-Nelson/SMS-co...
Relevant Papers:
support.illumina.com/ko-kr/bu....
/ a-look-at-the-nanopore...
hasindu2008.github.io/slow5sp...
github.com/hasindu2008/slow5t...
samtools.github.io/hts-specs/...
Course website: physiology.med.cornell.edu/fa...
X: / mason_lab
Негізгі бет Ғылым және технология Single Molecule Sequencing: File Formats [FAST5, POD5, BLOW5, SLOW5, FASTQ, SAM, BAM, VCF]
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