Description: m6a modifications are becoming an ever more important and recognized RNA modification important in RNA metabolic processes, including mRNA export and decay. We specifically review why Nanopore technologies, especially Oxford Nanopore direct-RNA sequencing, have revolutionized research surrounding m6a modification. We review a recent publication, linked below, in order to determine optimal software tools based on experimental design to perform transcriptome-wide m6a analysis.
The following questions, not exhaustive, are covered within the video:
1.) What are m6a modifications? Why are they important?
2.) By what mechanisms are m6a modifications influenced and influential?
3.) How have m6a modifications been detected with traditional short-read RNA sequencing?
4.) Why has Nanopore long-read direct-RNA sequencing provided better opportunities for detect these modifications?
5.) How were these tools evaluated and compared?
6.) What were the methodologies / datasets that the authors utilized in order to perform this analysis?
7.) What were some of the top-level results?
8.) What additional questions did the authors explore within the study text?
Article Link:
www.nature.com...
Additional Diagram Credits:
www.epigentek....
www.cell.com/f...
www.researchga...
GitHub Page:
github.com/zho...
Citation: Zhong, ZD., Xie, YY., Chen, HX. et al. Systematic comparison of tools used for m6A mapping from nanopore direct RNA sequencing. Nat Commun 14, 1906 (2023). doi.org/10.103...
Thumbnail Credit: www.abcam.com/
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