This lecture explains about the Two photon microscopy principle and advantages of using two photon microscope in imaging.
Like confocal microscopy, two-photon microscopy uses a laser to excite a fluorescent tag within a pattern and detectors to measure the emitted gentle. Nonetheless, unlike the lasers used for confocal microscopy, which provide single-photon excitation, the lasers utilized in two-photon microscopy excite by way of using near simultaneous absorption of two long wavelength (∼800 nm) photons.
Watch this video lecture to know the principle of the two photon microscopy with the instrumentation and the role of two photon microscopy.
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Thank you for watching the technique video on two photon microscopy principle.
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