Superb... Spike and recovery video also do that for vitamins and cholesterol
@MicroChemsExperiments
3 жыл бұрын
Ok we will do it. Stay with us.
@HugoMaihuiri
6 ай бұрын
Dear, could you share the methodology on which you based it please?
@SabahYousif-l7e
Жыл бұрын
hello, how are you what is the standard method please?
@saravanans101
10 ай бұрын
Hi ma'am I have one doubt Your using column is c18 it is phenomenex column or any other brands
@mohdabrar2839
3 жыл бұрын
wow thanks
@MicroChemsExperiments
3 жыл бұрын
You are welcome.
@EliElordi
Жыл бұрын
Hi, sorry but I have the question regarding the formula. I understand the calculation but somehow I'm confused on the dilution factor part, the dilution factor is mark up volume, mL divided with mass, g: hence in final calculation shouldn't the unit is still there? Meaning that we still have the ppm × ml/g unit? How can we just end up with ppm value since we haven't cancel out the ml/g unit. Please help me on this. Thanks a lot
@MicroChemsExperiments
Жыл бұрын
We found a unit from the dilution factor, which is ml/g. And the ppm means g/ml. So both units will cut each other.
@dr.ajaybarman7041
3 жыл бұрын
How u prepare the sample for analysis.. I have seen preperation of mobile phase, vitamin c standard preperation and running procedure of machines.. but not seen how sample is selected
Maam is the ç 2 is the concentration of vit c in 20 microL injected sample or amount of vitamin c in injected 20 microL sample
@MicroChemsExperiments
Жыл бұрын
C2 is concentration of vitamin c in your sample
@surjitdeka7092
Жыл бұрын
@@MicroChemsExperiments thank you
@queeniedalisay9970
2 жыл бұрын
Hi. Is there anyway to determine a assay of vitamins. Like titration/titrimetry
@MicroChemsExperiments
2 жыл бұрын
Yes. Tritrametric method is also available. We will upload later
@queeniedalisay9970
2 жыл бұрын
@@MicroChemsExperiments Cool! this could really help! thank you so much! Keep it up and God Bless. 🤩
@queeniedalisay9970
2 жыл бұрын
@@MicroChemsExperiments and other vitamins as well.
@saravanans101
10 ай бұрын
Ma'am please clear my doubt
@znng9762
Жыл бұрын
Can i determine the percentage of vitamin c in tomato paste please tell me ?
@MicroChemsExperiments
Жыл бұрын
Yes. You can
@mariamaniati8917
2 жыл бұрын
Hi! According to which authors is this mehod?
@MicroChemsExperiments
2 жыл бұрын
We combined several journal and modified in our lab to develop this suitable method.
@nitudutt3606
2 жыл бұрын
Good sir
@MicroChemsExperiments
Жыл бұрын
Keep watching
@surjitdeka7092
Жыл бұрын
Hello maam If I send my sample for quantification and method development to a institutions,should I provide methodology? They gave me the results but the peaks are not good.. They also charged 15k for method development because the methods i provided did not work.. If they have developed the method the peak should come good m I right sir Actually my sample was a formulation that I developed
@MicroChemsExperiments
Жыл бұрын
You shouldn't provide methodology to the analytical laboratory while you are paying for the test. Method development includes best quality of chromatogram with a sharp and nice peak.
@surjitdeka7092
Жыл бұрын
@@MicroChemsExperiments that means they are unable to develope the methods... Peaks are not sharp and this is my last semester..what should I d maam
@MicroChemsExperiments
Жыл бұрын
@@surjitdeka7092 Contact with them (Testing Service Provider)
@surjitdeka7092
Жыл бұрын
@@MicroChemsExperiments in case of broad peak can i calculate the concentration or not I have been in contact with the scientist of that institution. He tells that it's ok. They have run 3 times so no problem. I can put it in paper also
@surjitdeka7092
2 жыл бұрын
Maam if chromatogram show more peaks than the desired compund ..what does it mean . Can It gives the wrong result for the quantification of our desired compund. Please rply And is it necessary to get relative area percentage 100% for the quantification of the desired compound.
@MicroChemsExperiments
Жыл бұрын
Your chromatogram may contain many peaks with different retention time. You should consider only that peak of retention time which match with the retention time of standard peak
@surjitdeka7092
Жыл бұрын
@@MicroChemsExperiments thank you
@surjitdeka7092
Жыл бұрын
@@MicroChemsExperiments that mean we should only consider the peak that is match with our desired compund
@وليدويتا
Жыл бұрын
reference de methode svp😢
@MicroChemsExperiments
Жыл бұрын
No specific reference. It is a Laboratory developed in-house method
@lakhanyadav6205
3 жыл бұрын
Sir please make a video for gravimetric titration
@MicroChemsExperiments
3 жыл бұрын
Noted.
@mysoulis5267
3 жыл бұрын
Hello. Great job. Do you run fb page? İ' ve send you msg but there are not answer..
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