I've literally watched so many videos on this topic but this is the one that really made it stick. This video helped me so much for multiple reasons, with the first being the neat layout of the video. The second reason is relating the names of stuff to how it plays a role in the whole process, and even going as far as to explain "historical" context. Last but not least, the fact that you related the entire process to a real-life application made it a lot easier to understand how it all fits together, and it made me love it and genuinely want to understand it rather than just study it. Major thanks to you
@isurusrimadusanka8025
4 жыл бұрын
We have similar things watch this👇 kzitem.info/news/bejne/16h9l5yjsIiClIo
@tusharpathare7
4 жыл бұрын
Truly simplified.. One of the best KZitem channel for learners like me
Here are some notes too. kzitem.info/news/bejne/w6V4x6N_gXl9aZg___Polymerase Chain Reaction
@Fresh_K
5 жыл бұрын
I must confess that by far this is one of the best youtube channels I've come across.Its really #MEDSIMPLIFIED.Bless you.
@MEDSimplified
5 жыл бұрын
Thankk you for your awesome comments 😀
@alexandrahowell3142
3 жыл бұрын
HIV has an RNA genome, not DNA as you state at 6'06" of the video. For HIV, you'd first have to do reverse transcription to produce cDNA before you can do a PCR
@kushunadkat9087
2 жыл бұрын
Yes
@fathimahashim7005
2 жыл бұрын
But when this hiv virus enters the human cell, it starts to create dna for making further copies of themselves.
@alexandrahowell3142
2 жыл бұрын
The video is incorrect when it states "the blood of the patient contains the virus, and in the virus is its genetic material, the DNA". Rather, it should state, "the virus's genetic material which is a single stranded RNA molecule, is reverse transcribed within the cell it has infected, and the resulting DNA then integrates into the host cell chromosome".
@simeonikechukwu1957
2 жыл бұрын
Useful observation @Alexander Howell
@HelloAayush
2 жыл бұрын
@@alexandrahowell3142 but when hiv virus is already in macrophages then it's dna due to reverse transcription is already integrated in the macrophage cell and further in the T helper cells The viral RNA is converted to DNA in macrophages. So we can say that we will directly get the DNA integrated in the cell?🤔
@aysha744
3 жыл бұрын
Truly simplified I cant understand anymore and This topics are made me boaring But now it is very amazing Thanks a lot
@RaudiRaul
3 жыл бұрын
You say at 5:30 in the video that the temperature should be raised to 72 degrees Celsius for step one, Denaturation. Isn't it higher like around 95 degrees Celsius?
@gayatriconventschoolbaran7538
3 жыл бұрын
I m in love with ur voice it is soo calming soo soothy... Watching video of other teachers on you tube give me headache as their voice is soo noisy but listening to u is really relaxing... And ur presentation is superbb
@oyenimeapreala6460
3 жыл бұрын
ThankS to DR Rorpopor Herbal on KZitem who helps me cured my PCR God bless you DR Rorpopor Herbal his herbal medication is very infective and active
@updown7607
3 жыл бұрын
In my book temperatures mentioned in denaturation is 94 degrees Celsius and for 1 minute. In primer Annealing, temperature is 54 degrees Celsius and time duration is 2 minutes.
@nidhishreeg6292
3 жыл бұрын
Thank you so much for explaining this topic in such an easy and understanding way!!
@kritikagautam1140
2 жыл бұрын
So simplified and easy to understand. Brilliant Explanation. Thank you ❤
@latrellebrown7916
4 жыл бұрын
Thank you! Automation Engineer here trying to move to the next level of understanding the things that I do.
Thank you sir for trully clearing my all doubt of this topic.
@ilyassalmon9513
2 жыл бұрын
One of the best KZitem channel for Students like me
@dr.garima_gynae
2 жыл бұрын
Very simple explanation of a complex topic👍👍
@behappywithmanotg9379
3 жыл бұрын
Really easily understandable ,very informative
@basavaraj.bdoranalli9956
3 жыл бұрын
U r not making it simple....u r simply making others mind simple....about a particular topic,...osm work...must appreciate..your work ..all D best...,🤘🤘
@terrybash5194
3 жыл бұрын
Thank you so much DR RORPOPOR HERBAL on KZitem you saved my life from this deadly PCR virus, I got cured within 14daysly herpesly herpes
@favouritethings9363
3 жыл бұрын
Thank you very much for this video.It is very well explained ,simply and easy to remember
@bhinggo7255
5 жыл бұрын
Thank you MEDSimplified! You have always helped me in every biochem exam! Thank you for making our student lives easier! Bless you!
@aishwaryabhatnagar75
3 жыл бұрын
thank you for this..i needed a simple and accurate definition n functioning of pcr..for an interview..thank you
@TheOiler1989
4 жыл бұрын
Thanks a lot and I'll be back to learn more about this. Now I know why Kary Mullis called PCR a manufacturing process.
@yrahlabog9422
3 жыл бұрын
This is so helpful for my report. ❤️ Thanks a lot.
@zafranmalik8998
4 жыл бұрын
All med simplified videos are grt & nice
@ramzanrajput8161
3 жыл бұрын
one of the best youtube channels God bless you
@sahibaikram5484
3 жыл бұрын
hye, thank you so must for making this topic super easy. my English is not so good so please don,t mind. you are amazing. i am seventeen years old and i want to be a great doctor just like you. you are my top inspiration. thanks again
@MEDSimplified
3 жыл бұрын
Thank you so much for the appreciation
@apryLwitha_why
2 жыл бұрын
Hello. I'm from FLORIDA, USA. Your English is not bad at all. I understood you perfectly. I wish you much luck in your medical career as a Doctor. I'm sure you will do great things in this field.
@MunsKi
2 жыл бұрын
its primary use is genetic research... not infection diagnosis, it is only later used
@consolidate_de_dreamer
3 жыл бұрын
One min down in the video already subscribed🙌🏾, amazing explanation thank you!
@rozellas2210
2 жыл бұрын
The MOST INFORMATIVE AND SIMPLIFIED VIDEO EVER ON PCR! Thanks a lot!
@cjain646
4 жыл бұрын
Great job, Very helpful, good voice. Thank you
@MEDSimplified
4 жыл бұрын
Thanks for the appreciation •°
@cjain646
4 жыл бұрын
You really have an amazing voice, the person who is drawing does a good job too.
@Emjay_Music
3 жыл бұрын
You. Are . Saving. My. Semester. Thank god for you!!!
@MoyMoy-q6m
7 ай бұрын
So about the Process of PCR; Denaturation, is when the sample is heated, breaking the hydrogens bonds that holds the double-strand DNA together, resulting the separation of the double-stranded DNA; in Annealing, the sample is finally cooled down, allowing the primers to bind with the individual strands of DNA; in Extension, the primers produce more nucleotides of the short sequence of the primer and once a new DNA is completely formed, the double-strand DNA will also undergo the same steps repeatedly by 20 to 30 times, resulting the millions of copies of DNA. Correct me if I'm wrong.
@vocalistharshi
3 жыл бұрын
You are blessing for us sir You explanation is so simplified, 🤧🤧really really helpful , keep making 💜💜 Thanku 🙃
@koushikbiswas5803
Жыл бұрын
Your voice is awesome! God gifted!
@bubblysnowflake5869
4 жыл бұрын
Thanks a lot sir.
@saikeerthanarachamadugu8171
3 жыл бұрын
Your idea on flashcard is excellent
@lihiycohen7477
3 жыл бұрын
Wow thank you for this video!! Very well explained and presented. Super useful.
@jyotirachchh002
Жыл бұрын
Thank you 😄🤍
@michaeldaveapo7207
4 жыл бұрын
Your video is really relevant today for the COVID - 19 pandemic. It helps people understand the testing process of identifying a person that has COVID - 19
@sportysbusiness
4 жыл бұрын
You mean understanding that the test doesn't work? Even it's inventor said it should ever be used to test for viruses. No wonder it's 80% inaccurate, a coin toss is more accurate.
@daisytoromanzano8865
10 ай бұрын
@@sportysbusinessThose are facts, thanks for the reminder. Kary Mullis said that you can find whatever you want, just increased the cycles. He also said never to be use to diagnosed just for lab research. Also contains E.O. In UK under a FIOA disclosure it was found that has EO( carcinogenic agent), way above safe limits. We cant put trust in any man, just Abba.
@KoToKo90cHaN
2 жыл бұрын
Great video! Just wanted to note that you've mentioned two different temperatures for denaturation, 96 degrees at 4:50 and 72 degrees the rest of the video, which one is correct?
@parv9994
2 жыл бұрын
Sarah it is always 96 degrees for denaturation and 72 degrees for extension (a.k.a. elongation). He probably got confused during recording.
@trinaaaich3529
3 жыл бұрын
Thank u so so much. Was of great help.
@robertschlesinger1342
4 жыл бұрын
A worthwhile video for all beginning biology students.
@rizwan7540
Жыл бұрын
Thank you so much sir. It's a really informative and interesting video I have really seen. May Allah accept your struggle. Aameen . Love from Rizwan 🥰
@DrReem-bm1yg
3 жыл бұрын
Thank you ,, it is really really simple and clear explanation ^^
@Khaniofficialchannel
4 жыл бұрын
Super Clear voice & best explanation of PCR.Thumbs up ⚘
@ironshield4
3 жыл бұрын
Hi, I'd like to request clarification regarding one of the points covered in the video. You say there will be 2 copies after the first cycle, then 4 after the second, and 16 after the third, then 32, 64, 128, and 512. Unless I'm missing something, or misunderstood (which is entirely possible), I think the 3rd cycle should yield 8 copies, not 16. If I'm right, then all of the subsequent values are wrong as well. Additionally, 512 would follow 256, not 128. Otherwise, it's a very helpful video, and I appreciate the work you put into it. Thanks!
@JaswinderSingh-qi7iy
3 жыл бұрын
You are right .I was searching for this comment so as to confirm that I wasn't wrong
@yogeshlakshman8488
2 жыл бұрын
Thanks to clear my doubt
@duocphamtruongtho4462
2 жыл бұрын
Thank you so much!
@sunithatella1554
6 ай бұрын
Thank you, 👍 it's really superb explanation
@aditeayah
4 жыл бұрын
Thanks
@tharindunuwangamage8688
3 жыл бұрын
Good job guys
@shahbaloch4814
3 жыл бұрын
Grest method of teaching easily understand and learn it thanks a lot sir 😊
@stevemartin4249
3 жыл бұрын
One of many problems in governments using PCR for mass testing of the coronavirus - why is there no standardized number of cycles to be run to determine a positive or negative test for RNA snippets? It is as if business or political interests choose the results they want first, and then choose the number of cycles to give that result. Say it ain't so Joe.
@danielm.1521
3 жыл бұрын
Oh c'mon man...straight Malarkey! Take that mess to another comment section and quit spreading the nonsense and causing doubt. You don't like per results then go the antigen route.
@debravictoria7452
3 жыл бұрын
Diagnosis? The inventor, Kary Mullis said the PCR was of no use for diagnosis.
@TheFelltimber
3 жыл бұрын
You are confusing end time PCR with RT PCR. The latter is used for covid testing.
@godloves9163
3 жыл бұрын
@@danielm.1521 Get a life! What an ignorant response. kzitem.info/news/bejne/t41ryIKaZnZhnaw
@kardusbox6430
2 жыл бұрын
Look at the Indonesian PCR business..
@faisalfida9929
2 жыл бұрын
Best For Non Medicals😍😍
@adabwaleobonato5561
Жыл бұрын
Very useful information and well defined...one thing mistake is here an example of Virus HIV containing DNA...🧬
@crypted4527
4 жыл бұрын
Please make a video on Recombinant DNA technology
@pratikbagthariya7089
3 жыл бұрын
Thank you this is the best channle for remember biology 🙏❤️👍💯
@Slarti
3 жыл бұрын
My understanding is that the UK is using around 45 cycles for detecting sars-cov-2 which sounds way over the top given this video.
@TheFelltimber
3 жыл бұрын
This is end time PCR...which is not used for covid testing
@MastermindTV
2 жыл бұрын
Thank goodness i found you
@niks8924
5 жыл бұрын
Thanks so much for this vedio...i easily understood the PCR
@MEDSimplified
5 жыл бұрын
Thank you so much
@ravikanttyagi9633
4 жыл бұрын
Is this best to detect abdominal tuberculosis
@deemamattar517
2 жыл бұрын
This video is literally amazing 😍😍
@sunithath799
2 жыл бұрын
Thank you it's really simple to understand.
@Rayyanbinshad
5 ай бұрын
In my book its written that in danaturation template is heated to 94 degrees and you said 72 degrees
@tonywong7300
3 жыл бұрын
This is an eye opening explanation. Thanks
@dhunganalaxmi8277
Жыл бұрын
Wow what a teaching style ❤❤❤
@shaiestamalik5226
4 жыл бұрын
Very well explained.. Please share a vedio of Rtpcr procedure for Covid-19..
@sandeep.v8589
3 жыл бұрын
Really MED is simplified
@Roselynne1016
4 жыл бұрын
Can you explain why Kary Mullis the inventor of the PCR test stated over and over that the PCR test should not be used to diagnose viruses?
@Roselynne1016
4 жыл бұрын
@John Bindini , thank you.
@torgenxblazterzoid
4 жыл бұрын
@jorge pearl Nobody has isolated and/or purified COVID-19. If you believe otherwise then post your *definitive* evidence here.
@boilingfrog783
4 жыл бұрын
@@shellysistla9037 - Hi, I'm a complete layman so please excuse any ignorance on my part. On the subject of the Covid virus, what medical conclusions would you draw from PCR positives where the cycle threshold has been set high - 40 cycles or more - when the video indicates less cycles should be performed (Professor Carl Heneghan has indicated 25 as appropriate) and the test subject is clearly asymptomatic? Is it correct to refer to such a person as a "case" and is there any realistic possibility of them being infectious? Thanks. Best regards 👍
@alvarodias68
3 жыл бұрын
@John Bindini I understand that pcr shouldn't be used to diagnose illness. But why do you want people to kiss your ass?
@alvarodias68
3 жыл бұрын
@steven atkinson Bingo!
@sameerquazi2626
2 жыл бұрын
96 degrees is the temperature at which DNA denatures, not 72. Once denatured aat 96, annealing of primers to strands takes place at 56 dgerees and then Taq Polymerase comes into action at 72.
@mireillemvondo-she8884
2 жыл бұрын
Thank you so much, I really appreciated the easy explanation.
@profiliomongjam1556
3 жыл бұрын
Thank you very much for explaining pcv in simple way
@loopeesa1823
11 ай бұрын
It's so simple and realatable!
@sturdybirdyy
Жыл бұрын
Great video. But the temperature for each step could be cross checked. Plus one copy will make 2 then 4 then 8, not 16 as stated in the video. Another thing that you mentioned was that after the first cycle there is one parenteral DNA and one newly formed..it should be that there are 2 copies each with one parental strand and one newly synthesized strand..
@hadich3009
2 жыл бұрын
jazakAllah hu khaira
@jiniaisrat9434
4 жыл бұрын
Thank you so much. I was searching an easy video. It helped me a lot.
@lidhiyalouis1851
3 жыл бұрын
Excellent presentation
@House_of_wealth
2 жыл бұрын
Thank you for this video, it really help me a lot
@nazzia1
3 жыл бұрын
please waiting for the video on agarose gel electrophoresis. Your videos are amazing
@RiazKhan-og7bf
4 жыл бұрын
Good for learning in easy way but many questions can still arises here
@shikhakumari2212
3 жыл бұрын
Very nice explanation sir👍👍👍👍
@medicinestudent8349
2 жыл бұрын
Thank you!
@libertyfirst4083
2 жыл бұрын
Does PCR actually "diagnosis" infections? I thought it could tell you whether a sample has viral DNA, but only that. It can't tell you whether someone is "infectious", correct? For that, you'd need to take a "positive" sample and put into a cell culture to see if the virus infected those cells. If that happens, the person is both "positive" for virus and "infectious". Correct? If the cells do not become infected, then the person is not infectious. It's really a two-step process as _part_ of a full clinical diagnosis of illness. Simply having "the virus" tells you very little, yes?
@florashlaks
2 жыл бұрын
How is the primer decided? I mean, is the base pair sequence same in all primers? What decides which primer to use?
@aqsakauchali5674
3 жыл бұрын
Sir I have one question Primer gets bind to the specific site of DNA n then the process starts but what about the other site where primer did not bind..?
@hazaraudi7488
Жыл бұрын
That site doesn't need to be amplified
@riazkhattak527
2 жыл бұрын
Thank you so much sir 😊😊
@pasqualefasciano6592
3 жыл бұрын
If they never isolated Sars-cov 2 to use how primer, how and why are they using PCR test to determinate if a person tests positive or negative for an infectious disease that apparently is never been isolated before?
@stevemartin4249
3 жыл бұрын
Exactly. Dr. Sam Bailey's KZitem asks the same question. Another question ... why is there no standardized number of cycles to be run to determine a positive or negative test for RNA snippets? It is as if business or political interests choose the results they want first, and then choose the number of cycles to give that result.
@nawrasal-hasanat3643
2 жыл бұрын
Thanks a million
@janicecarman3157
2 жыл бұрын
Salamat pangsagot ko sa biotech 🥰
@janicecarman3157
2 жыл бұрын
Well come
@alieucham3391
3 жыл бұрын
The temperature "90-95°C" in denaturing, not 72°C,
@-Shrinath
Жыл бұрын
Would like to get a video from regarding agarose gel electrophoresis, sturtevant's obsevation, Morgan's linkage theory, etc..
@dr.maliksiddiqui6203
3 жыл бұрын
Sir HIV is a retrovirus having RNA as genetic material but you said that HIV having DNA as genetic material. Kindly correct it.
@Alpha-Omega33
2 жыл бұрын
I don’t understand how the multiplication of the DNA shows if you have a certain decease or not? This is what I mostly want to know and it’s still not clear.
@sujitdey1717
4 жыл бұрын
Quite helpful... Thank you for making this video...
@nehavikram007
16 күн бұрын
Finally... something made sense
@likilkumarsunkari3025
2 жыл бұрын
Well explained bro
@mychannel4397
3 жыл бұрын
Very good and helpful video
@satyaranjanacharya4635
Жыл бұрын
u know u voice is just 😍😍
@Sami-khan33
Жыл бұрын
Very helpful
@katejbradshaw
3 жыл бұрын
Well done!
@tanzeelahashim8168
3 жыл бұрын
Good explanation 👍👍
@AwaisAli-xl1pu
2 жыл бұрын
Really impressive thumbs up
@pallemsaishivani1861
4 жыл бұрын
Thank you soo much for your amazing tutorial sir 🥰🥰
@karenjwoods5961
3 жыл бұрын
Thank you very clear explanation..now i can understand
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