This video covers details on Real Time PCR Assays- Taqman Assay Vs SYBR Green Assay, Differences, Advantages and Disadvantages.
Real Time PCR differs from the conventional PCR/ End point PCR is that in real time PCR both amplification and quantification of the target DNA molecule is possible.
In real time PCR the amplified DNA is detected in real time, where as in convention PCR detection is possible only at the end of PCR by performing agarose gel electrophoresis for the PCR product.
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