i dont know why i am studying at a university WHEN THESE CHANNEL ARE HERE ON KZitem . really ggood
@laetitiaf.2671
6 ай бұрын
I totally agree
@azca.
2 жыл бұрын
I'm suspecting this will be one of those channels that will be incredibly successful in years to come. Growth will be slow especially on KZitem, but if you continue producing incredibly informative and quality explanations like this, I'm sure you will go far. You explained this concept perfectly. Thank you!
@YouTooBio
2 жыл бұрын
Thank you for the lovely compliment! I'm glad my video was helpful.
@BustinStephen
Жыл бұрын
The MIQE guidelines published in Clinical Chemistry in 2009 clarify this issue: RT-PCR is reverse transcription PCR. Real-time quantitative PCR is qPCR.
@abineshanbazhagan6703
Жыл бұрын
Being new to PCR and stuff, I was really finding it confused , especially after going through the literature. But here? It's a wow, never I had come across of such an explanation which not just cleared my confusions but paved a way for some novel research ideas. Ton of thanks. Warm appreciations and looking for more of this kind. Thanks again. Good day.
@GabeThinks
2 жыл бұрын
Incredibly clear, engaging, and informative video! I needed a quick refresher and I now am totally comfortable with the concept of RT-PCR and qPCR. Thank you for making this!
@YouTooBio
2 жыл бұрын
Thanks! 🥰
@debjitbanerjee8512
3 жыл бұрын
Please make more such amazing videos.
@YouTooBio
3 жыл бұрын
Thanks! Will do
@Jesusandbible
Жыл бұрын
the RT PCR Test used for Covid-19 - Though it is easy to trace the discovery of Reverse transcriptases to Howard Temin , and its independently isolation by David Baltimore in 1970, and it is also very easy to identify the inventor of underpinning PCR Test as Kary Mullis, I have yet to identify a scientist or group of them that take the credit for each of the RT PCR Test (real Time vs. Reverse Transcription). Do you know? Does anyone know?
@mahdiachbar3756
2 жыл бұрын
Just WOW ! Excellent explanation really !! Thank you❤️❤️
@YouTooBio
2 жыл бұрын
Thank you!! 🥰
@AW-yb5qy
Жыл бұрын
Do you have a video explaining amplification techniques such as FRET, TaqMan, Molecular Beacon, and Scorpion Probe? HELP!
@roseviolet6522
Жыл бұрын
Hocam mala anlatır gibi anlattığınız için teşekkür ederim anladım sonunda
@Aston_Tom
9 ай бұрын
Sorry if I sound incorrect here, but through explaining Reverse Transcription PCR you explain how when you have regular DNA at the cDNA stage, does that imply you have left the Uracil in it, with not using RNAse H and Extending the DNA from the fragments to create a double stranded copy DNA of the original mRNA?
@falloutmodder
2 ай бұрын
Thank you for this excellent video, very clear and helpful! Excellent help studying for my Microbiology final!
@prabalmajumder5179
2 жыл бұрын
You made it crystal clear mam ..thanks a lot . please make more explanatory vdos
@kaylacraddock8670
Жыл бұрын
Helloo, so we know that DNA polymerase cannot use RNA as a template to build a complementary strand. What is the biological reason therefore? Is it due to structure or direction of activity?
@richardsarabi2064
Жыл бұрын
You are not showing on your graph the number of copies. Like you said ten versus 10000 is not shown on the graph. I get the guicker plateau in fewer cycles and that of course means more copies for that sample, but it's not plotted on the y axis.
@monenga
2 жыл бұрын
Thank you so much! You are the best explainer ever. Plus, you have such great energy and demeanor, you are also fun and pleasant to watch and to listen to. I hope you are a teacher!
@YouTooBio
2 жыл бұрын
Thanks! 😊 I am a teacher!
3 ай бұрын
OMG. Loving your explanation (and thanks for it), the music, and the dynamic of the video.
@dr.mousumidasgoswami5089
2 жыл бұрын
Wonderful explanation
@aousshaheen3239
10 ай бұрын
Very profitable video thank you ❤
@alinaaleman4264
3 жыл бұрын
Great video, easy to understand! thanks
@YouTooBio
3 жыл бұрын
Glad to hear!
@ayleenc.205
10 ай бұрын
So can you use primers for specific genes that code specific proteins and then use RT PCR in both its meanings to quantify how much there is of each mrna?
@anacarolina1578
4 ай бұрын
i guess so, because that's exactly what i'm doing in my masters degree haha
@YolandaWilliams-d2y
Күн бұрын
7586 Hahn Wells
@soosyr1996
7 ай бұрын
I owe you :( super thank you
@tommytruong4255
2 жыл бұрын
This is super helpful. Thank you so so much for doing this
@YouTooBio
2 жыл бұрын
Glad it was helpful!
@jusocampo
3 ай бұрын
great vid thank u!!
@tusarbehera6359
Жыл бұрын
🎉🎉You are good a teacher
@alisasj2382
Жыл бұрын
Thank you very much for the simple and concise explanation
@EmersonTiffany
29 күн бұрын
962 Nikolaus Forge
@GreenWard-d5m
29 күн бұрын
6160 Orn Fork
@amagaming2585
Жыл бұрын
shere material please
@somoshreetaluqqder3706
Жыл бұрын
Extremely thankful. Wonderful explanation.
@bozhang6135
7 ай бұрын
Amazing video! Keep up the great work.
@jennifersmith677
Жыл бұрын
This was so helpful. Highly recommend!
@deresekia7344
Жыл бұрын
Everything here is lovely explained, thanks!
@Nooruddinsadiq
11 ай бұрын
Thanks ❤
@ΑδαμαντίαΨευτογκα
Жыл бұрын
great video! its a talent to explain so complicate things, so easy! Congrats!
@FredAtwood
26 күн бұрын
959 Schmeler Ports
@laetitiaf.2671
6 ай бұрын
Thank you for the explanation. They are really clear
@josephakinkunmi
7 ай бұрын
this was excellent. thank you.
@xxdeadqueenxx5715
2 жыл бұрын
Thank you so much for this!! I needed to understand how exactly real time pcr worked for a case study I have to present in front of pathologists for school.
@dereksniper
7 ай бұрын
good use of real life examples
@ranaelnady4128
Жыл бұрын
AMAZING THANK U SO MUCH!
@saraabdulaziz3764
Жыл бұрын
Thank you so much for this!!
@synapticsarah
Жыл бұрын
Amazing example of scientific communication!! Thank you from a fellow science communicator.
@YouTooBio
Жыл бұрын
Always special to get a compliment from another science communicator!
@malihaaneeqa2343
Жыл бұрын
The best exaplanation so far
@elibenaron
Жыл бұрын
underrated video
@David_Hogue
Жыл бұрын
I love how easy this makes it to understand
@thesuperiorbench6307
Жыл бұрын
Cheers Jamie :D
@halilahnordin37
2 жыл бұрын
After complete reverse transcriptase and real time pcr,how we test the sample, validating it has sars cov 2?
@YouTooBio
2 жыл бұрын
If there is PCR product after reverse-transcription PCR, then there was SARS-CoV-2 in the original sample. If there is no PCR product, then there was no SARS-CoV-2 in the original sample.
@christianutida1589
2 жыл бұрын
thank you so much!
@kaylabrandt2992
2 жыл бұрын
Thank you for such an informative and educational video! This helped me learn the difference in RT-PCR and PCR for my Virology exam tomorrow!
@YouTooBio
2 жыл бұрын
Great! Glad it was helpful!
@beatrizsfsilva
Жыл бұрын
Amazing content!
@sara97979
2 жыл бұрын
awesome
@ahmadhegazy7811
3 жыл бұрын
can you recommend for me a book to understand the extraction of alkaloids or extraction in general? and thanks
@YouTooBio
3 жыл бұрын
Interesting question! Unfortunately alkaloid extraction is outside my area of expertise, so I can't make specific recommendations. :(
@ahmadhegazy7811
3 жыл бұрын
@@YouTooBio Ok. So, any recommendations about practical books in your field?
@YouTooBio
3 жыл бұрын
@@ahmadhegazy7811 I can't recommend specific books in my field - we tend to learn techniques through direct mentorship from other grad students or postdocs in the lab. However, if you're looking for step-by-step video tutorials, definitely check out Jove www.jove.com/
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