How does H. Influenzae grow on the sheep blood or horse blood, even though it doesn't have hemolysis ability to access the hemin (Factor X) inside the blood?
@patricktracy9947
2 жыл бұрын
Hi Mays, On the quad plate, the sheep/horse blood quadrant is used to detect hemolysis, not growth, so it has both V and X factors. By the way, in a sheep blood plate, there is extra-cellular X factor. The V factor is intracellular in the RBCs, so it can only be utilized after the RBCs have been lysed and the V factor released. Chocolate agar is boiled blood agar and therefore it has both V and X factors available for usage. That is why Haemophilus influenzae grows on chocolate agar.
@keitholiver4771
2 жыл бұрын
Did you use one colony to inoculate each quadrant or did you use 4 different loops for each quadrant
@patricktracy9947
2 жыл бұрын
Touching a single colony will give plenty of organism to inoculate all four sections.
@missknowall
2 жыл бұрын
Just discovered this wonderful series of videos. Pity the channel appears abandoned.
@patricktracy9947
2 жыл бұрын
No, it's not abandoned.
@missknowall
2 жыл бұрын
@@patricktracy9947 great to hear that. Please keep these wonderful videos coming.
@cvc2465
4 жыл бұрын
What is the purpose of the Choc plate?
@patricktracy1057
4 жыл бұрын
The package insert states that the blood quadrant should only be used for hemolysis, not growth, therefore the chocolate is needed to detect H. influenzae and H. hemolyticus.
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