This is exactly what I was looking for! Thank you sir for this masterpiece!!
@BioLabCollective
4 ай бұрын
Thanks for watching!
@emmaportnoy
Ай бұрын
What amount of DNA should be loaded to each well?
@luissantos2355
2 жыл бұрын
the level of production is amazing. how do you not have more views?
@BioLabCollective
2 жыл бұрын
The algorithm is tricky to figure out, but glad you were able to find the channel!
@DrHaywheezie_Neurotutes
2 ай бұрын
Absolutely Amazing
@freespirit6855
2 ай бұрын
Do we need serial dilution for running a PCR?
@BioLabCollective
2 ай бұрын
Not always, depends on what you are trying to dilute out? Same principles apply when you are setting up your PCR mastermix and diluting it out for the different reactions.
@fatcammal
7 ай бұрын
This is THE most thorough and effective PCR video i've ever seen
@BioLabCollective
7 ай бұрын
Thank you so much! Glad it helps
@skazka3789
Жыл бұрын
Hello again Professor! With regards to the PCR, the machine says there is an amplification in my negative controls. But the CT values for the negative controls are consistently 10 points higher than the CT values for the actual samples. For example CT value of 37 for negative control vs CT of 21 for the sample. These results are still fine yeah?
@BioLabCollective
Жыл бұрын
I'm not really sure about your experimental context so can't really say by CT values alone. Have you run the samples on a gel to assess the size of the bands? Probably the next thing to try
@siasmith6746
3 ай бұрын
this is the clearest, most precise video I have seen on PCR. I normally do not comment but this was phenomenal
@siasmith6746
3 ай бұрын
I am a subscriber now, all of your videos are made with such care.
@BioLabCollective
3 ай бұрын
Thank you! Took a long time to make, glad the effort shows on screen
@emmadegroote4014
Жыл бұрын
Thank you, this helped sooo much!!! You're videos are amazing!
@BioLabCollective
Жыл бұрын
Glad they help - thank you for taking the time to comment!
@NaveenKumar-it3gq
10 ай бұрын
Thank you for the simplified lesson. Keep up the good work.
@BioLabCollective
10 ай бұрын
Thanks for watching and commenting!
@InquilineKea
Жыл бұрын
OH THE COMB CREATES HOLES IN THE GEL
@BioLabCollective
Жыл бұрын
The gel lanes (or holes) need to be standard sizing all the way across - a comb makes that happen more or less
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